The roster of guest stars on Route 66 includes several actors who later went on to fame, as well as major stars on the downward side of their careers. One of the most historically significant episodes of the series in this respect was "Lizard's Leg and Owlet's Wing." It featured Lon Chaney, Jr., Peter Lorre and Boris Karloff as themselves, with Karloff donning his famous Frankenstein monster make-up for the first time in 25 years and Chaney reprising his role as the Wolfman. The show was filmed at the O'Hare Inn, near O'Hare Airport, Chicago, Illinois. Dutch singer Ronnie Tober had a small guest role with Sharon Russo, Junior Miss America.